Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: FABP4 regulates eosinophil recruitment and activation in allergic airway inflammation

doi: 10.1152/ajplung.00429.2017

Figure Lengend Snippet: Fatty acid binding protein 4 (FABP4) promotes cell adhesion via regulation of integrin-β2 expression. A and B: adhesion and morphology of wild-type (WT) and FABP4 knockout (KO) eosinophils on recombinant murine (rm) VCAM-1-coated coverslips. C and D: adhesion and morphology of WT and FABP4 KO eosinophils on rm ICAM-1-coated coverslips. Scale bar = 20 µm in B and D. E: rolling of WT and FABP4 KO eosinophils on rm VCAM-1-coated coverslips under conditions of flow (wall shear stress 1.0–2.0 dynes/cm2) in a flow chamber. F: detachment of WT and FABP4 KO eosinophils from rm ICAM-1-coated coverslips under conditions of flow in a flow chamber. G: expression of cell adhesion molecules in WT and FABP4 KO eosinophils by flow cytometry. H: expression of peroxisome proliferator-activated receptor-γ (PPAR-γ) in WT and FABP4 KO eosinophils by qPCR. Combined data (means ± SE) of 3 experiments in duplicate are shown in A, C, E, F, and H. Representative data of 3 independent experiments with eosinophils from 3 different mice/group are shown in B, D, and G. *P < 0.01 in A, C, and F and <0.05 in H for comparison with WT eosinophils.

Article Snippet: Briefly, an equal number of WT and FABP4 KO eosinophils were allowed to adhere to recombinant murine (rm) coated vascular cell adhesion molecule (VCAM-1; Leinco Technologies, Fenton, MO)-coated coverslips or rm intercellular adhesion molecule (ICAM-1; Leinco Technologies)-coated coverslips for 30 min at 37°C.

Techniques: Binding Assay, Expressing, Knock-Out, Recombinant, Shear, Flow Cytometry, Comparison